The DIMO uses the internal illumination source for transmission optical microscopy. With the fluorescence option, light from an optical fiber can be easily guided into the CoreAFM for epi-illumination from the bottom.
AFM data can be overlaid with the optical data to colocalize information from each imaging mode. The image shows an overlay of AFM topography with fluorescence (green) and transmission light (gray) optical microscopy images. The overlay was created with MountainsMap image processing software.