Digital Inverted Microscope Option

Integrated inverted microscope functionality for CoreAFM

  • View samples from below
  • Exchangeable objective lens
  • Enhances FluidFM® functionality

The CoreAFM is a very versatile atomic force microscope that can be used for a multitude of different types of research. The digital inverted microscope option (DIMO) enhances your CoreAFM to allow optical view from below, replacing the need for a completely different AFM setup with an inverted microscope.

Enhanced viewing ability

The DIMO empowers you to conduct advanced experiments that require an inverted microscope, as is the case for many Bio AFM applications. It enhances your capabilities when using accessories like the petri dish holder (visible at the beginning of the video to the right), and does not obstruct any standard accessories that fit your CoreAFM (with the exception of the variable magnetic field sample holder).

Fluorescence measurements

The DIMO uses the internal illumination source for transmission optical microscopy. With the fluorescence option, light from an optical fiber can be easily guided into the CoreAFM for epi-illumination from the bottom.

AFM data can be overlaid with the optical data to colocalize information from each imaging mode. The image shows an overlay of AFM topography with fluorescence (green) and transmission light (gray) optical microscopy images. The overlay was created with MountainsMap image processing software.

Even better with FluidFM® functionality

EasyLift’s low-drift, high-precision movements allow simple and consistent creation of traditional TEM lamella or ultra-thin lamella using the inverted thinning technique. The EasyLift is integrated with the microscope’s xT software to provide a simple, intuitive method for lift-out and transfer of TEM samples to a grid, all within the DualBeam chamber. Highly accurate and fast motorized rotation makes the EasyLift ideally suited for high speed inverted or plan view sample preparation. Functionality includes simple “click and drag” movement enabled by EasyLift’s full integration with the Helios NanoLab 1200AT DualBeam’s user interface.

(Left) Optical image of cantilever with attached colloid taken with DIMO.
(Right) Elastic modulus of HeLa cell measured with FluidFM® colloidal spectroscopy

Motorized function for cantilever centering and focus

FluidFM® spotting observed by DIMO

High-powered microscope optics

  • Objective: 20× 0.4 NA long working distance objective from Nikon
  • Objective is interchangeable between experiments with other Nikon objectives that have a M25×0.75 thread and 60-mm parfocal length
  • Camera: Highly sensitive 2.3 MP monochrome CMOS camera with 5.86 µm × 5.86 μm pixel size (eq. 1/1.2”), USB 3.0, frame rate up to 48 frames/s at full size
  • Fluorescence option with fiber coupling and filter set that can be changed between experiments
  • Filters are in a Nikon filter block, as used for Eclipse Ti series microscopes
  • Optical resolution: 0.8 µm with default objective and transmission
  • Motorized focusing and cantilever centering

0.8 μm resolution with 20× 0.4 NA objective shown on USAF 1951 Resolving Power Test Target