Krios G3i Cryo-TEM

Unravelling ‘Life’ at the Molecular Level – Easier, Faster, and more Reliably

The new Thermo Scientific™ Krios™ G3i Cryo Transmission Electron Microscope (Cryo-TEM) enables life science researchers to unravel life at the molecular level—easier, faster, and more reliably than ever before. Its highly stable 300 kV TEM platform and industry-leading Autoloader (cryogenic sample manipulation robot) are designed for automated applications, such as single particle analysis (SPA) and cryo-tomography. Designed-in connectivity ensures a robust and risk-free pathway throughout the entire workflow, from sample preparation and optimization to image acquisition and data processing.

Setting up data acquisition has been made easier and quicker by enhanced automation and systematic user guidance. This allows every user to achieve the ultimate performance for every experiment. Simultaneously, the high-resolution performance and throughput of the Krios G3i Cryo-TEM have been further improved by cleverly combining hardware improvements with advanced software capabilities.

Key Benefits

Enhanced ease of use through automation

The Krios G3i Cryo-TEM reduces the number of complex alignments that a user has to perform. For recurring alignments that need to be done regularly, automated alignment routines have been implemented into the EPU software for SPA. Furthermore the EPU user interface has been further streamlined to provide comprehensive user guidance.

Reproducible, optimal tool performance always guarantee

The optimal thermal and mechanical stability of the Krios G3i Cryo-TEM ensure perfect optical performance. The instrument features a self-assessment function that automatically evaluates the optical status of the microscope, providing feedback for any steps that require optimization. Additionally, automated alignment routines allow the instrument to be tuned to its optimal starting point for SPA or tomography experiments.

Maximum Throughput

EPU is the native software package for SPA automated screening and acquisition of large data sets on the Thermo Fisher cryo-TEMs. With full control of the Autoloader from within EPU, all grids in a cassette can be batch-screened: after the creation of a grid atlas, ice quality (presence, thickness) of the vitrified grids is automatically categorized to support guided selection of grid squares.

High resolution performance

The Krios Cryo-TEM has a proven track record of high resolution imaging of a wide variety of particles: the vast majority of published structures at or below 4 Å have been determined using Thermo Fisher cryo-TEMs . Constant power lenses reduce thermal drift, and contribute to the excellent system stability during long automated acquisition sessions. To allow imaging of increasingly smaller particles at increasingly higher resolution, the Krios G3i comes with Volta Phase Plate integration and a guaranteed <1% anisotropic magnification distortion.

Workflow connectivity

For successful cryo-EM data acquisition, optimization of both biochemistry and vitrification requires an efficient screening process. The Krios G3i Cryo-TEM can be integrated in a SPA workflow to where samples will be evaluated and optimized using the Talos Arctica or Glacios Cryo-TEMs, before imaging them at high-resolution in the Krios G3i Cryo-TEM

The designed-in connectivity of the Autoloader capsule and cassette systems ensure a robust and contamination-free transfer of samples between Glacios, Arctica and Krios Cryo-TEMs, without the need for manipulation of individual small grids.